Asuragen’s DiscovArray miRNA Expression Profiling Service
Asuragen’s DiscovArray miRNA Expression Profiling Service utilizes an Ambion array,
manufactured by Affymetrix. The DiscovArray miRNA Expression Service offers the
most content, with probes for verified Sanger miRNAs and more than 12,000 predicted
miRNAs, allowing you to interrogate a dramatically larger number of putative miRNAs
with robust, qualified procedures.
Learn more about how we qualify our methods and staff.
Exploratory
- Validated and novel content representing >13000 miRNAs [Probe
Content] and [Performance
Specs]
- Sanger 9.2 (annotated to current Sanger Version)
- Migration of novel content to validated with every Sanger update
Unique
- Superior labeling technology requires less input (100ng) and provides
industry-leading sensitivity and specificity
- All miRBase organisms represented
Interactive
- miRInform™,
Asuragen-developed Data Delivery System
- Publication-ready figures and tables
Description of Services
Sample Prep services:
- RNA isolation methods verified to recover miRNA
- Incoming total RNA QC (sample
preparation services):
- Quantitation and estimation of RNA purity using NanoDrop spectrophotometer readings at 260 and 280 nm
- qRT-PCR assessment of discrete miRNAs as a surrogate measure of overall
miRNA abundance
- See our Sample Submission and Shipping Instructions for recommended incoming sample QC
values
- Ambion/Affymetrix for DiscovArray services:
Deliverables
As part of the DiscovArray miRNA Expression Profiling Service, you will receive
Asuragen’s miRInform™
Data Delivery System containing your data in an easy-to-understand package
with these benefits:
- Optimized for miRNA analysis
- Fast, cost-effective analysis for a wide range of experimental
designs
- Accessible DVD format
- High quality figures linked directly to the Sanger miRBase
- Easy to sort tables
To ensure security, all information is provided in an electronic form on a DVD.
Encryption is available on request. Data may also be delivered by FTP or e-mail.
Quality Control
During our processes, we monitor many QC checkpoints. As much as possible, we utilize
spike-in controls, in-process sample controls, and process or reagent batch controls.
Should any sample-specific QC measure result in an “alert” or “fail” reading, we
will contact you to discuss available options. These rigorous procedures enable
Asuragen to be the only service provider to offer a
Data Quality Guarantee:
- If, for any reason, a qualified sample does not pass our process control
specifications, we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
standardization.
Turn-around Time
Most projects are completed in one to two weeks.
Contact us if you have a critical time requirement; we can often meet your
need for no additional charge.
Read more about our commitment to prompt turn-around times.
Additional Services Available from Asuragen
Catalog Numbers for miRNA DiscovArray Services
Sample Prep
|
CA0032
|
QC, NanoDrop and TaqMan, Total RNA, for microRNA Applications
|
|
CA0041
|
Isolation, Total RNA from cell pellet
|
miRNA Expression
|
CA0032
|
QC, NanoDrop and TaqMan, Total RNA, for microRNA Applications
|
|
CA0044
|
Array Processing and Scanning, Affymetrix DiscovArray Process
|
|
CA0050
|
Target Preparation, miRNA, Affymetrix DiscovArray
|
Data Analysis
|
CS0010
|
Data Analysis, Custom
|
|
CS0020
|
miRInform™ Data Delivery System for DiscovArray or Agilent miRNA Expression
Profiling
|