Applied Biosystems TaqMan® Platform
Asuragen is a licenced provider for all Applied Biosystems TaqMan assays and arrays*.
Services Available
Asuragen offers cGLP/CLIA-registered services on all AB TaqMan products
Technology
mRNA
TaqMan Gene Expression Assays and Custom TaqMan Gene Expression Assays are built
on AB’s 5′ nuclease chemistry and consist of a FAM™ dye-labeled TaqMan MGB probe,
and two unlabeled PCR primers. All components are QC tested by Applied Biosystems.
TaqMan Arrays
The TaqMan Array is a 384-well micro fluidic card, built on the TaqMan
technology, that enables you to perform 384 simultaneous real-time PCR reactions
without the need to use liquid-handling robots or multichannel pipettors to fill
the card. This low- to medium- throughput micro fluidic card allows for 1-8 samples
to be run in parallel against multiple TaqMan Gene Expression Assay targets that
are pre-loaded into each of the wells on the card.
microRNA (miRNA)
The basis of the TaqMan MicroRNA Assays is a target-specific stem-loop structure,
reverse-transcriptase primer. Its innovative design overcomes a fundamental problem
in miRNA quantitation: the short length of mature miRNAs (~22 nt) prohibits conventional
design of a random-primed RT step followed by a specific real-time assay. The stem-loop
accomplishes two goals: 1) specificity for only the mature miRNA target, and 2)
formation of a RT primer/mature miRNA-chimera, extending the 5′ end of the miRNA.
The resulting longer RT amplicon presents a template amenable to standard real-time
PCR, using TaqMan Assays. To ensure accurate results, every individual TaqMan MicroRNA
Assay design has been functionally validated under laboratory conditions.
TaqMan miRNA Assays are not only specific for mature miRNAs, they can also successfully
distinguish between highly homologous targets1.
TaqMan miRNA Assays are extremely sensitive— researchers need only 1–10 ng of purified
total RNA or equivalent to reliably quantify their miRNAs of interest, not the several
micrograms typically required for hybridization based methods.
Quality Control
During our processes, we monitor many QC checkpoints as recommended by the Applied
Biosystems’ procedures. As much as possible, we utilize spike-in controls, in-process
sample controls, and process or reagent batch controls. Should any sample-specific
QC measure result in an “alert” or “fail” reading (such as a standard deviation
of triplicate qRT-PCR readings exceeding defined limits), we will contact you to
discuss available options. These rigorous procedures enable Asuragen to be the only
service provider to offer a
Data Quality Guarantee:
If, for any reason, a qualified sample does not pass our process
control specifications, we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
standardization.
Sample Handling and Processing
All samples are received and entered into the analysis queue under a controlled
SOP to confirm package/sample integrity, appropriate shipping conditions (frozen
samples/dry ice, as appropriate), and individual confirmation of sample identity
against the shipping manifest and electronic sample
submission form and
instructions.
Turn-around Time
Most projects are completed in one to two. If you have a critical time requirement,
we can often meet your need for no additional charge.
Read more about our commitment to prompt turn-around times.
Related Services
Data Analysis
Analytical assay validation and CLIA-based testing services also are available.
Catalog Numbers for TaqMan qRT-PCR Services
Gene Expression, mRNA - Single tube Assays and Arrays
|
CA0030
|
AB TaqMan Service for mRNA, single assays, RT-PCR
|
|
CA0035
|
TaqMan Assay Validation, mRNA
|
|
CA0046
|
AB TaqMan Arrays for mRNA, PCR only
|
|
CA0047
|
RT for TaqMan mRNA Array, random-primed
|
miRNA
|
CA0032
|
QC, NanoDrop and TaqMan, Total RNA, for miRNA applications
|
|
CA0028
|
AB TaqMan Service for miRNA, single assays, RT-PCR
|
|
CA0042
|
AB TaqMan Service for miRNA, single assays, RT-PCR , absolute quantitation
|
*This test is performed pursuant to license arrangements with Roche Molecular Systems, Inc. and Applera Corporation.
1Chen
C,
Ridzon DA,
Broomer AJ,
Zhou Z,
Lee DH,
Nguyen JT,
Barbisin M,
Xu NL,
Mahuvakar VR,
Andersen MR,
Lao KQ,
Livak KJ,
Guegler KJ (2005). Real-time
quantification of microRNAs by stem-loop RT-PCR.
Nucleic Acids Res 33(20):e179.