AmplideX™ FMR1 PCR*
The AmplideX® FMR1 PCR (RUO) reagents are state-of-the-art research tools for the detection of CGG repeats
in the fragile X mental retardation (FMR1) gene. This product supports two different PCR formats,
Gene-specific FMR1 PCR and CGG Repeat Primed(RP) PCR. These reagents can detect full mutations up to at
least 1300 CGG, accurately size up to 200 CGG repeats, detect interrupting AGG sequences and resolve female
zygosity. These reagents have been evaluated in three peer review publications and tested in dozens of laboratories in the US and abroad.
Either PCR format can reduce the number of samples
required for Southern blot analysis by as much as
50-fold*.
Traceability to Fragile X Testing Standards
The AmplideX® FMR1 PCR (RUO) reagents are traceable to the Fragile X panel materials available from the National Institute of Biological Standards and Control (NIBSC).
This panel, certified by the World Health Organization as an international standard, comprises female normal, female pre mutation, femal full mutaion, male full mutation and male pre mutation with
repeats ranging from 22 to 759 CGG. NIBSC Fragile X Reference Material
Novel PCR Configurations
Asuragen currently recommends the following instrumentation for use with these PCR
reagents:
- ABI 9700 Thermocycler, ABI Veriti, or MJ Research PTC-200 thermal cyclers
- An ABI Multicapilary Genetic analyzer (3130, 3730, 3500) running POP-7 Polymer
Use of instrumentation outside of these recommendations cannot be supported at this
time.
For more information, please send your inquiries to fragilexbd@asuragen.com.
*Research Use Only. Not intended for use in diagnostic procedures.